There is great potential to use ultrasound and microbubbles for treating cardiovascular disease such as thrombosis and acute myocardial infarction. However, progress toward clinical practice of these therapies has been hindered by a lack of full understanding of transient ultrasound and microbubble behavior at the target site. Our group designed and constructed a novel ultra-fast bright-field microscope capable of imaging dynamics of therapeutic ultrasound and microbubbles in vitro at speeds up to 200 million frames per second with 2 nanosecond image exposure times. An overview of the instrument is described including illumination design, optical aberration characterization and image post-processing. Instrument spatiotemporal accuracy and precision are discussed and example image sequences are presented. These data show an ultra-fast bright-field microscope capable of studying ultrasound-driven microbubble behavior in vitro, providing unique insight into mechanisms of microbubble-mediated therapeutic ultrasound for cardiovascular disease.
|Title||An ultra-fast bright field microscope for studying behavior and effects of therapeutic ultrasound and microbubbles for cardiovascular disease|
Knight Cardiovascular Institute, Oregon Health & Science University, Portland, Oregon 97239, United States of America
|Session||7. Biomedical applications|
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